Composite

Part:BBa_K3320015

Designed by: Joseana Mendes Comodaro   Group: iGEM19_Amazonas-Brazil   (2019-10-20)


J23100 + lldR

Second part of the lactate repressor unit from our AND Gate. Constitutive production of Lactate Repressor.

Usage and Biology

This part produces constitutively the LldR, which represses the promoter from BBa_K3320014 in absence of lactate. LldR binds to two operator sites, O1 and O2, with O1 being filled at a lower concentration of LldR. Binding of L-lactate to LldR promotes a conformational change that may disrupt the DNA loop, allowing the formation of the transcription open complex.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None